dapi solution thermofisher 62248 Search Results


4 6 diamidino 2 phenylindole dapi  (Thermo Fisher)
99
Thermo Fisher 4 6 diamidino 2 phenylindole dapi
4 6 Diamidino 2 Phenylindole Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
4 6 diamidino 2 phenylindole dapi - by Bioz Stars, 2026-09
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dapi  (Thermo Fisher)
99
Thermo Fisher dapi
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution+thermofisher+62248/PBS/pmc11285089-494-12-13
Average 99 stars, based on 1 article reviews
dapi - by Bioz Stars, 2026-09
99/100 stars
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4 6 diamidino 2 phenylindole  (Thermo Fisher)
99
Thermo Fisher 4 6 diamidino 2 phenylindole
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
4 6 Diamidino 2 Phenylindole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+solution+thermofisher+62248/4'%2C6'-DIAMIDINO-2-PHENYLINDOLE+DIHYDROCHLORIDE/pmc05915663-136-6-9
Average 99 stars, based on 1 article reviews
4 6 diamidino 2 phenylindole - by Bioz Stars, 2026-09
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4’,6-diamidino-2-phenylindole dihydrochloride  (Kyfora Bio)
93
Kyfora Bio 4’,6-diamidino-2-phenylindole dihydrochloride
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
4’,6 Diamidino 2 Phenylindole Dihydrochloride, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
4’,6-diamidino-2-phenylindole dihydrochloride - by Bioz Stars, 2026-09
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tetrodotoxin citrate  (Alomone Labs)
95
Alomone Labs tetrodotoxin citrate
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Tetrodotoxin Citrate, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
tetrodotoxin citrate - by Bioz Stars, 2026-09
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superfrost plus slides  (Fisher Scientific)
86
Fisher Scientific superfrost plus slides
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Superfrost Plus Slides, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
superfrost plus slides - by Bioz Stars, 2026-09
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choline chloride  (Enamine Ltd)
99
Enamine Ltd choline chloride
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Choline Chloride, supplied by Enamine Ltd, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
choline chloride - by Bioz Stars, 2026-09
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sb 431542  (Bio-Techne corporation)
99
Bio-Techne corporation sb 431542
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Sb 431542, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
sb 431542 - by Bioz Stars, 2026-09
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vectashield antifade mounting medium with dapi  (Vector Laboratories)
98
Vector Laboratories vectashield antifade mounting medium with dapi
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aif-1/iba1 antibody  (Bio-Techne corporation)
98
Bio-Techne corporation aif-1/iba1 antibody
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Aif 1/Iba1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horse serum  (EuroClone)
90
EuroClone horse serum
(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: <t>DAPI</t> (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation <t>of</t> <t>Tg(ddx5:DDX5-GFP)</t> transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.
Horse Serum, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: DAPI (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation of Tg(ddx5:DDX5-GFP) transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.

Journal: Cell reports

Article Title: Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties

doi: 10.1016/j.celrep.2023.112787

Figure Lengend Snippet: (A and B) Presence and location of predicted PrD (A) and intrinsically disordered region (IDR; B) in DDX5 in killifish and humans. Red line: PLAAC score. DDX5 amino acid positions are indicated at the bottom. Amino acid composition is color coded. Blue line: local disorder score (DISOPRED3). (C) Immunohistochemistry for DDX5 in brain sections from young (3.5 months) and old (7 months) male killifish. Green: DDX5; blue: DAPI (nuclei). Image representative of 3 individual fish for each age group. Staining was performed twice independently, ~7 sections per fish per experiment. Scale bar: 10 μm. Quantification of the subcellular localization of DDX5 puncta is in . (D) Immunohistochemistry for DDX5 in brain sections from young (4 months) and old (28 months) male mice. Green: DDX5; blue: DAPI (nuclei). Image representative of 2 mice per age group, 6 sections per mouse. Scale bar: 20 μm. (E) Generation of Tg(ddx5:DDX5-GFP) transgenic F0 founders (there was no germline transmission). (F) Immunohistochemistry for GFP in brain sections from old (~6–7 months) male Tg(ddx5:DDX5-GFP) F0 founders (which can have variable protein expression). Green: GFP; blue: DAPI (nuclei). Image representative of 3 individual fish, ~6 sections per fish. Scale bar: 10 μm. Immunocytochemistry in human 293T cells for untagged killifish DDX5 full length (red, antibody to killifish DDX5) or GFP-tagged killifish DDX5 (full length [FL] and two different truncation mutants [ΔIDR and ΔPrD] [green, antibody to GFP]). Left: representative images from two independent experiments, each performed in triplicate. White arrows indicate cells with puncta (determined by signal intensity over a pre-defined maximum). Scale bar: 10 mm. Right: mean ± SD of the percentage of transfected cells showing DDX5 puncta. Each dot represents the average of ~240 cells from 3 fields of view over two independent experiments. Circles: experiment #1; triangles: experiment #2. p values: Kruskal-Wallis with Dunn’s correction.

Article Snippet: For GFP-tagged constructs, cells were washed 3 times with PBS, incubated with DAPI (ThermoFisher, 62248) (1:1000 in PBS) for 20 min at RT and washed 2 times more before imaging.

Techniques: Immunohistochemistry, Staining, Transgenic Assay, Transmission Assay, Expressing, Immunocytochemistry, Transfection